Stacker - GreenHouse Work-Up Station

Stacker - GreenHouse Work-Up Station

Rapid sequential and parallel purification in a 24 well micro titer plate footprint using standard filtration, phase separation and SPE columns...

The Stacker allows two levels of filtration, phase separation and/or SPE (solid Phase Extraction) columns to be stacked one above the other. This enables parallel and sequential purification of 24 samples into standard 3ml and 6ml tabbed or tables columns. The principle of the Stacker allows efficient purification of samples from any standard 24 well format micro titer plate, as well as the GreenHouse reaction blocks, 24 x 7ml micro titer plates and other 24 position reactors/synthesisers.

• Stacked column design allows sequential purification e.g. phase separation and SPE or filtration and SPE
• Easily removable upper support plate allows unit to be used for single or sequential parallel purification
• Clear gas cover allows slight positive pressure to be applied to increase flow rate of difficult samples
• Design accommodates all standard 3ml and 6ml tabbed or tables SPE or filtration columns
• Accepts all standard 24 well micro titer plates, as well as GreenHouse Reaction Blocks for fraction collection

Unique recessed design of support plates allows positioning of standard format columns within micro titer plate footprint without column tabs obstructing each other

Solid Phase Extraction (SPE) Columns
Silica most useful for purifying moderately polar compounds where the impurities are either or both very non polar (elute first) or very polar (retain on column)

Aminopropyl the amino group can hydrogen bond with a variety of polar groups, e.g. hydroxyl, amino etc and will have polar interaction with polar groups. It will also function as a weak anion exchanger when protonated.

SCX a strong cation exchanger used to extract basic compounds capable of holding a positive charge. Can be used for extracting basic impurities from non-basic product, which pass straight through.

SAX Acetate a strong anion exchanger used to extract compounds capable of carrying a negative charge (e.g. weak acids). Can be used for extracting acidic impurities from non-acidic products, which pass straight through.

Filtration Columns 20µm PE
Typical applications for filtration columns are isolation of precipitates or crystallites from reaction mixture. Filtration of resins prior to cleavage, filtration of solid supported reagents post synthesis. Removal of solid impurities prior to SPE. Pressure can be applied to individual column using SpeedFlow to increase the flow rate, particularly if the column becomes blocked.

Phase Separation Columns
Phase separation columns contain a frit and are designed to separate organic phases from water. Heavier than water (Chloroform, dichloromethane etc) or lighter than water (diethyl ether, hexane etc) . It should not be necessary to apply pressure, indeed doing so can allow the aqueous layer to break through the hydrophobic frit.

Liquid/liquid Extraction Columns
Unlike Phase Separation Columns these Liquid/liquid extraction columns can be used with non-water miscible solvents that are either more or less dense than water. When a two phase mixture is applied to the column the aqueous phase is absorbed onto the diatomaceous earth and the organic phase runs through the column. The high surface area at the interface between the organic and the aqueous phase increases efficiency and eliminates the possibility of emulsion formation.